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KMID : 0370220120560010014
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Yakhak Hoeji
2012 Volume.56 No. 1 p.14 ~ p.19
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Evaluation of Oxidative Stress and Antioxidant Enzyme Expression in Human Hepatocarcinoma SK-Hep-1 Cells Treated with Stearic Acid
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Oh Jung-Min
Lee Ji-Yoon
Lee Gwan-Ho
Kim Bong-Hee
Kim Sang-Kyum
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Abstract
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Nonalcoholic steatohepatitis(NASH) is serious metabolic disease related to fatty acid. According to "two hit theory", fatty acid-induced oxidative stress is important factor to progress nonalcoholic steatohepatitis from steatosis. In this study, we evaluated stearic acid induced oxidative stress in human hepatocarcinoma SK-Hep-1 cell. Cell viability, reactive oxygen species (ROS) production, glutathione (GSH), malondialdehyde and expression of antioxidant enzymes were determined at various time-points and concentrations of stearic acid. At 0.2 mM, non-toxic concentration, of stearic acid, production of ROS was significantly increased at 24 hours and the level of GSH was significantly decreased. Expression of superoxide dismutase-1 and 2 was slightly increased in 0.2 mM stearic acid at 24 hours. These results represent that the non-toxic concentration of stearic acid resulted in oxidative stress, suggesting that stearic acid may play a critical role in development of steatohepatitis.
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KEYWORD
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NASH, oxidative stress, stearic acid, steatosis, saturated fatty acid
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